Figure 6.

Dynamic protease activities during seed germination. A, Proteome conversion during seed germination. Proteins were extracted from germinating seeds at different time points in duplicate and detected on Coomassie Brilliant Blue-stained protein gels. RBCL, Rubisco large subunit. B to D, Dynamics of protease activities during germination. Protease activities were displayed by labeling protein extracts with FY01 (B), JOGDA1 (C), and JOPD1 (D). For all experiments, seeds were imbibed for 2 d on agar plates at 4°C in the dark and germinated in 16 h of light for 3 d.