Table III. Mass spectrometry quantification of PSI and PSII subunits in chromatography eluates from different genotypes.

Relative quantification by mass spectrometry-based label-free quantification of PSI and PSII subunits in affinity chromatography eluates is shown. All quantifications are normalized to the content in psbs KO. Relative enrichment in PSBS-expressing genotypes (N-His-PSBS/C-His-PSBS) is also shown. LHCBM, LHCB4, and LHCB5 include multiple polypeptides that were not individually distinguished, as detailed in “Materials and Methods.” n.d., Protein not detected. All data are expressed as averages ± se of three independent replicates.

Uniprot Accession	Annotation	N-His-PSBS	C-His-PSBS	psbs KO	N-His-PSBS/C-His-PSBS
Q6YXN7	PSBA	1.94 ± 0.43	0.90 ± 0.18	1	2.16 ± 0.37
Q6YXM8	PSBB	2.56 ± 0.55	0.92 ± 0.19	1	2.77 ± 0.47
Q6YXN9	PSBC	2.07 ± 0.32	0.88 ± 0.19	1	2.34 ± 0.35
Q6YXN8	PSBD	2.43 ± 0.50	0.78 ± 0.02	1	3.11 ± 0.37
A9T914, A9SGM1, A9S6S7, A9TL35, A9RT62, Q765P5, A9RJS3	LHCBM	5.69 ± 1.35	1.85 ± 0.07	1	3.07 ± 0.43
A9TKU6	LHCB3	6.85 ± 1.55	1.90 ± 0.15	1	3.60 ± 0.50
A9U3M1, A9T2F8	LHCB4	8.48 ± 3.90	1.66 ± 0.30	1	5.12 ± 1.46
A9U5B1, A9RFL7	LHCB5	3.73 ± 1.23	1.17 ± 0.13	1	3.18 ± 0.64
A9RU32	LHCB6	4.04 ± 0.71	1.81 ± 0.64	1	2.23 ± 0.51
A9TED6	LHCSR1	2.21 ± 0.21	n.d.	1
A9T399	LHCA1.1	4.51 ± 0.87	1.48 ± 0.08	1	3.05 ± 0.35
Q8MFA3	PSAA	1.95 ± 0.22	1.02 ± 0.13	1	1.92 ± 0.19
Q8MFA2	PSAB	2.89 ± 0.53	1.08 ± 0.21	1	2.68 ± 0.41
Q6YXQ2	PSAC	4.03 ± 0.74	1.90 ± 0.50	1	2.11 ± 0.39