Figure 2.

RT-PCR analysis of ZCN7 and ZCN8 transcripts in line B73 and teosinte plants. A, RT-PCR was performed with oligo(dT)-primed cDNA prepared with RNA extracted from mature (ML) and immature (IL) leaves and with (+) or without (−) the addition of reverse transcriptase (RT). Schemes of ZCN7 and ZCN8 genes and positions of primers used for RT-PCR are reported. The structures of both genes are conserved in line B73 and teosinte. B, Strand-specific RT-PCR carried out using forward (ZCN7-for and ZCN8-for) and reverse (ZCN7-rev and ZCN8-rev) primers for synthesizing cDNA produced by the antisense and sense RNA strands, respectively. Subsequent RT-PCR with ZCN7/8-1 and ZCN7/8-2 primers permits the detection of both spliced and unspliced RNAs. C, Diagram schematizing the three transcript isoforms produced by ZCN7 and ZCN8 genes. RNA length and position with respect to the gene structure are based on strand-specific RT-PCR results with line B73 plants.