Figure 3.

A, Optimized multielement chromatogram. Size exclusion profiles recorded for the nonoxide ions ²⁴Mg⁺ and ⁵⁵Mn⁺ and the oxide ions of ⁴⁸SO⁺ and ⁷²FeO⁺. Individual PSII subfractions (Mn₁, Mn₂, and Mn₃) and the PSI fraction (Fe₁) were collected. B, UV-Vis elution profiles measured at 280 and 663 nm together with the ²⁴Mg⁺ ion intensity signal (mAU, Milli absorption units). C, BN-PAGE gel of PSII subfractions (Mn₁, Mn₂, and Mn₃) followed by western blotting using antibodies against the D1 and CP43 subunits. Lane T represents barley thylakoids and was included as a size marker. An equal volume was loaded on each lane. SC, Supercomplex. D, Western-blot analysis with antibodies against PsaF, D1 and CP47 core proteins of PSII, extrinsic subunit PsbO, and antenna proteins Lhca1 and Lhcb1 of Mn₁ and Fe_l subfractions and barley thylakoids (lane T); 2.5 μg of total protein was loaded on each lane.