Gene structure and characterization of the mutant for CHLSYN. A, Gene structure of CHLSYN and T-DNA insertion site in SALK134433 (chlsyn1-1). Black bars represent exons, and lines indicate introns. The white triangle indicates the T-DNA insertion site. B, Plants shown are wild-type (WT) and null mutant chlsyn1-1, which were genetically and transcriptionally confirmed by PCR and RT-PCR, respectively. For RT-PCR analysis, the UBC gene (At5g25760) was used as an internal control.