Tocopherol accumulation is reduced in mature seeds of chlsyn1-1. Seedlings and seed morphologies (A and B) of chlsyn1-1 plants with genetic complementation of AtCHLSYN. Also shown are green seeds lacking DsRed fluorescence corresponding to noncomplemented seeds (C, left), seeds with DsRed fluorescence corresponding to complemented seeds (C, right), and comparison of tocopherol concentrations in seeds with chlsyn1-1 complementation (D; red) and noncomplemented chlsyn1-1 (D; nonred) seeds. Data represent means ± se of three independent biological replicates. Wt, Wild type; **, P < 0.01 (Student’s t test).