Sense suppression line accumulates CHLSYN-specific siRNAs. A, Plants shown from left to right are the wild type (Wt), sense suppression (Wt/CHLSYN), rdr6, and rdr6 transformed with CHLSYN (rdr6/CHLSYN) under control of the CaMV35S promoter. Also, RT-PCR analysis of CHLSYN transcript levels is shown in B. Shown in C, top is the accumulation of CHLSYN
siRNA (21–22 nucleotides) in leaves from Wt and rdr6 plants nontransformed or engineered for CaMV35S-mediated overexpression of CHLSYN. C, bottom shows ethidium bromide staining of low-molecular weight RNA.