Figure 1.

BiFC visualization of the target interaction partners in cv BY-2 tobacco cells under 514-nm excitation. The counterpart proteins were tested in a pairwise fashion by fusing the full-length genes separately to each of the N- and C-terminal fragments of YFP. The transformed cells were incubated at 23°C for 20 h in the dark and then partially plasmolyzed. Six pairwise combinations, any two proteins of StvacINV1, StInvInh2B, SbSnRK1α, and SbSnRK1β, are presented. No fluorescence was observed when any gene was coexpressed with empty vector or when PKIN1 (an α-subunit of one of the two potato SnRK1s identified) was coexpressed with StvacINV1. The test was conducted with three biological replicates. Bars = 20 μm.