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In vitro phosphorylation of the kinase and its potential substrates. Auto, Autoradiograph; CB, Coomassie Blue staining; KD, kinase domain of SbSnRK1α (amino acids 1–271); KUD, kinase and ubiquitin-associated domains of SbSnRK1α (amino acids 1–329). The results show that no protein in the StvacINV1-StInvInh2B-SbSnRK1 complex could be phosphorylated by SbSnRK1.
