Figure 6.
Relative expression levels, chip colors, and invertase activities of wild-type cv E3 and different SbSnRK1α transgenic lines. A, Quantitative real-time (RT)-PCR analysis revealed that SbSnRK1α was overexpressed in the tubers of overexpression lines (OE) and suppressed in the tubers of RNA interference lines (Ri). The expression level in wild-type cv E3 was taken as 1 for calculating the fold change in SbSnRK1α expression of each transgenic line. The transgenic tubers were compared with wild-type cv E3 separately for 4°C and 20°C storage. B, Invertase activity of potato tubers. FW, Fresh weight. C, Correlation between SbSnRK1α transcript level and invertase activity. Data from transgenic tubers stored at 4°C for 30 d were used. *, P < 0.05; **, P < 0.01 by Student’s t test. D, Color of potato chips from tubers stored at 4°C or 20°C for 0 d (0D), 15 d (4C15D and 20C15D), and 30 d (4C30D and 20C30D).