Table I. Effects of combinations of SbSnRK1 subunits and StInvInh2 on StvacINV1 activity.
| No. | Protein Mixturea | StvacINV1 Residual Activityb | Significancec |
|---|---|---|---|
| 1 | StvacINV1 | 1.000 ± 0.024 | a |
| 2 | StvacINV1 + SbSnRK1α | 1.057 ± 0.134 | a |
| 3 | StvacINV1 + SbSnRK1β | 1.001 ± 0.112 | a |
| 4 | StvacINV1 + SbSnRK1α + SbSnRK1β | 0.986 ± 0.109 | a |
| 5 | StvacINV1 + StInvInh2B | 0.422 ± 0.101 | b |
| 6 | StvacINV1 + StInvInh2B + SbSnRK1α | 0.430 ± 0.047 | b |
| 7 | StvacINV1 + StInvInh2B + SbSnRK1β | 0.949 ± 0.033 | a |
| 8 | StvacINV1 + StInvInh2B + SbSnRK1α + SbSnRK1β | 0.938 ± 0.091 | a |
a
The target proteins were mixed in 0.01 m phosphate-buffered saline (PBS) buffer where SbSnRK1α was not phosphorylated. bEach activity value is the mean ± se of five biological replicates, which is calculated by the StvacINV1 activity with or without other component protein(s)/the StvacINV1 activity measured when it presented alone. cDifferent letter presents significance at P = 0.05 by Student’s t test.