Table II. StvacINV1 residual activity in different protein mixtures.

No.	Protein Mixturea	StvacINV1 Residual Activityb	Significancec
1	StvacINV1	1.000 ± 0.069	a
2	StvacINV1 + GRIK1	1.005 ± 0.077	a
3	StvacINV1 + SbSnRK1α	1.016 ± 0.132	a
4	StvacINV1 + SbSnRK1α + GRIK1	1.000 ± 0.125	a
5	StvacINV1 + KD	1.032 ± 0.091	a
6	StvacINV1 + KD + GRIK1	0.988 ± 0.101	a
7	StvacINV1 + KUD	0.907 ± 0.080	a
8	StvacINV1 + KUD + GRIK1	0.954 ± 0.096	a
9	StvacINV1 + SbSnRK1β	1.039 ± 0.104	a
10	StvacINV1 + SbSnRK1β + SbSnRK1α	1.090 ± 0.122	a
11	StvacINV1 + SbSnRK1β + SbSnRK1α + GRIK1	0.948 ± 0.090	a
12	StvacINV1 + SbSnRK1β + KD	1.005 ± 0.024	a
13	StvacINV1 + SbSnRK1β + KD + GRIK1	0.999 ± 0.051	a
14	StvacINV1 + SbSnRK1β + KUD	1.000 ± 0.091	a
15	StvacINV1 + SbSnRK1β + KUD + GRIK1	0.979 ± 0.062	a
16	StvacINV1 + StInvInh2B	0.412 ± 0.011	b
17	StvacINV1 + StInvInh2B + SbSnRK1α	0.427 ± 0.035	b
18	StvacINV1 + StInvInh2B + SbSnRK1α + GRIK1	0.498 ± 0.046	b
19	StvacINV1 + StInvInh2B + KD	0.422 ± 0.059	b
20	StvacINV1 + StInvInh2B + KD + GRIK1	0.453 ± 0.042	b
21	StvacINV1 + StInvInh2B + KUD	0.407 ± 0.095	b
22	StvacINV1 + StInvInh2B + KUD + GRIK1	0.461 ± 0.050	b
23	StvacINV1 + StInvInh2B + SbSnRK1β	1.027 ± 0.060	a
24	StvacINV1 + StInvInh2B + SbSnRK1β + SbSnRK1α	0.930 ± 0.045	a
25	StvacINV1 + StInvInh2B + SbSnRK1β + SbSnRK1α + GRIK1	0.423 ± 0.073	b
26	StvacINV1 + StInvInh2B + SbSnRK1β + KD	0.967 ± 0.035	a
27	StvacINV1 + StInvInh2B + SbSnRK1β + KD + GRIK1	0.470 ± 0.068	b
28	StvacINV1 + StInvInh2B + SbSnRK1β + KUD	0.923 ± 0.061	a
29	StvacINV1 + StInvInh2B + SbSnRK1β + KUD + GRIK1	0.449 ± 0.050	b

^aThe target proteins were mixed in kinase reaction buffer that supplied ATP for kinase activation. KD and KUD are different regions of SbSnRK1α.  ^bEach activity value is the mean ± se of five biological replicates, which is calculated by the StvacINV1 activity with or without other component protein(s)/the StvacINV1 activity measured when it presented alone.  ^cDifferent letters indicate significance at P = 0.05 by Student’s t test.