Figure 5.

HDA6 binds to the promoter regions of ETC1 and GL2. A, Schematic representation of selected regions for ChIP-PCR analyses. B, Enrichment of HDA6 at the promoter regions of ETC1 and GL2 was tested by ChIP-PCR. Transgenic plants bearing the 35S_pro:HDA6-myc construct were subjected to ChIP assays with an anti-myc antibody. Col plants were used as negative controls. ACTIN7 and TUBULIN2 (TUB2) were used as an internal control for normalization. Error bars represent sd values from at least three biological replicates each consisting of three technical replicates. **, P < 0.01 and *, P < 0.05, Student’s t test.