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. 2015 Jul 4;168(4):1448–1458. doi: 10.1104/pp.15.00821

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Figure 6. — HDA6 affects histone acetylation of the ETC1 and GL2 promoter regions. A, Histone H3 acetylation status at selected promoter regions of pattern genes in axe1-4 was analyzed by ChIP assay using antibodies specific to acetylated histone H3K9K14 followed by quantitative PCR. ACTIN7 was used as an internal control for normalization. Error bars represent sd values from at least three biological replicates each consisting of three technical replicates. **, P < 0.01 and *, P < 0.05, Student’s t test. B, Histone H4 acetylation status at selected promoter regions of pattern genes in axe1-4 was analyzed by ChIP assay using antibodies specific to acetylated histone H4K5K8K12K16. ACTIN7 was used as an internal control for normalization. Error bars represent sd values from at least three biological replicates each consisting of three technical replicates. **, P < 0.01, Student’s t test. TUB2, TUBULIN2.