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. 2015 Aug 8;8:413. doi: 10.1186/s13071-015-1028-6

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© Moraes et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Intracellular Ca2+ release (a) by mononuclear (MN) cells indicated by geometric mean fluorescence intensity of Fluo-3. MN cells were pre-incubated with cytokines or left untreated. Intracellular Ca2+ release (b) after 2 h of incubations. *indicates statistically significant differences between MN cells incubated with cytokines and the control [PBS]. Cells were stained with Fluo-3 [Fluo3-Acetoxymethyl], and immunofluorescence analyses were carried out by flow cytometry [FACScalibur, Becton Dickinson, USA]