Figure 2.

The systematic design of the smear-based BCS1. The illustration demonstrates the complete factorial systematic design of the initial PEG smear-based screen BCS1. The buffers were used at a concentration of 0.1 M and the additives were (i) potassium acetate (KOAc), (ii) lithium sulfate (Li₂SO₄), (iii) ammonium nitrate (NH₄NO₃), (iv) lithium chloride and magnesium chloride mixture (LiCl + MgCl₂) and (v) ethylene glycol, all at 0.2 M concentration apart from ethylene glycol, which was at 10%(v/v). The PEG smears used are low molecular weight (LMW), medium molecular weight (MMW), high molecular weight (HMW) and broad molecular weight (BMW).