Table 2. Examples of proteins crystallized in the BCS2 screens.

Proteins	Abbreviation	UniProt ID	MW (kDa)	Examples of hits	Smear precipitants	Single PEG precipitants from primary screens
Acetyl-CoA carboxylase 1 (C-terminal domain)	ACACA	Q13085	87.4	23, 65, 89, 92	MMW, BMW	3350, 5K MME
Amphiphysin (bar domain)	AMPH	P49418	24.2	2, 14, 20, 37, 39, 40, 42, 45, 66, 68, 78, 80, 96	LMW, MMW, HMW, BMW	3350
Bromodomain adjacent to zinc-finger domain protein 2B (bromodomain)	BAZ2B	Q9UIF8	13.6	3	LMW	600, 1K, 6K
ATPase family AAA domain-containing protein 2 (bromodomain)	ATAD2	Q6PL18	15.4	12, 14, 22, 31, 40, 41, 43, 44, 45, 47, 50, 54, 57, 61, 62, 63, 65, 66, 74, 78, 79, 80, 81, 92, 93	LMW, MMW, HMW, BMW	1K, 3350, 6K, 8K, 20K
Bridging integrator 2 (N-bar domain)	BIN2	Q9UBW5	28.0	3, 9, 10, 11, 15, 17, 21, 23, 26, 27, 33, 27, 33, 38, 39, 47, 49, 59, 82, 84	LMW, MMW, HMW, BMW	3350, 10K
Cyclin-dependent kinase-like 5 (kinase domain)	CDKL5	O76039	35.2	12, 18, 27, 38, 39, 48, 63, 65, 67, 83, 89	LMW, MMW, HMW	20K
DNA cross-link repair 1A protein	DCLRE1A	Q6PJP8	41.3	92	BMW	1K, 3350, 10K
5 Exonuclease apollo	DCLRE1B	Q9H816	37.8	50, 53, 61, 66	LMW, MMW, HMW	400
Dual-specificity tyrosine phosphorylation-regulated (kinase 1A kinase domain)	DYRK1A	Q13627	41.9	39, 94	MMW, BMW	300, 400, 3350
Zinc phosphodiesterase ELAC protein 1	ELAC1	Q9H777	40.7	85, 5, 80, 35, 65	LMW, MMW, BMW	3350, 4K
Mitogen-activated protein kinase 1 with inhibitor	ERK2VTX-11e complex	P28482	41.5	28, 47	MMW
ETS translocation variant 1	ETV1DNA complex	P50549	12.4	5, 31, 39, 40, 49, 52, 53, 55, 57, 62, 68, 73, 78, 79, 81, 83, 84, 85, 89, 92, 93, 96	LMW, MMW, HMW, BMW
Guanylate cyclase soluble subunit -1	GUCY1B3	Q02153	24.0	40, 83	MMW	1K, 3350
Glycogenin 1	GYG1	P46976	29.6	1, 4, 8, 11, 13, 14, 15, 18, 19, 22, 23, 25, 32, 33, 37, 43, 46, 52, 53, 55, 56, 57, 58, 59, 60, 67, 68, 69, 70, 71, 72, 73, 79, 80, 81, 82, 83, 84, 85, 86, 91, 92, 93, 94, 95, 96	LMW, MMW, HMW, BMW	1500, 3350, 2K MME, 8K
Kelch-like protein 2	KLHL2	O95198	30.3	9, 20, 43, 65, 66, 70, 74, 79, 80, 86, 88, 89	LMW, MMW, HMW, BMW	400, 3350, 4K, 5K MME, 6K, 8K
-Lactamase-like protein 2	LACTB2	Q53H82	32.9	21, 27, 41, 52, 59, 64, 76	MMW, HMW, BMW	2K MME, 3350, 4K, 5K MME, 6K, 10K
Methionine adenosyltransferase I	MAT1A	Q00266	43.7	33, 85	LMW, BMW	3350
Methylmalonyl-CoA epimerase, mitochondrial	MCEE	Q96PE7	14.4	3, 4, 5, 8, 10, 12, 14, 15, 20, 24, 26, 28, 32, 33, 38, 39, 40, 46, 47, 59, 60, 64, 68, 72, 82, 83, 89, 92, 96	LMW, MMW, HMW, BMW	3350, 4K, 8K, 10K
Malonyl-CoA decarboxylase, mitochondrial	MLYCD	O95822	50.4	3, 9, 22, 80, 81, 82, 92	LMW, MMW, BMW	3350, 5K MME, 6K, 8K, 10K, 20K
P300/CBP-associated factor (bromodomain)	PCAF	Q92831	14.2	11	MMW	3350, 10K
Membrane-associated tyrosine- and threonine-specific CDC2-inhibitory kinase	PKMYT1	Q99640	32.0	9, 41, 50, 62, 65, 67, 70, 76, 79, 85, 88, 89, 94	LMW, MMW, HMW, BMW	1K, 3350
Tumour protein p73-like (tetramerization domain)	TP73L	Q9H3D4	7.4	10, 87, 32, 53, 40, 19, 68, 76, 4, 25, 87, 3, 82, 54, 81, 56, 27, 26, 37	LMW, MMW, HMW, BMW	550 MME, 1K, 3350, 4K, 5K MME
GDP-L-fucose synthase	TSTA3	Q13630	35.3	3, 15, 38, 60	LMW, HMW	3350, 4K, 6K, 10K
MAP kinase 14 complexed with TAB1-activating peptide	p38TAB1 complex	Q16539	44.4	9, 75	MMW	3350
Cyclin-G-associated kinase (kinase domain)	GAKnanobody complex	O14976	53.8	15, 22	HMW, BMW	3350, 5K MME, 10K
RAS-association domain-containing protein 3	RASSF3	Q86WH2	16.2	47	MMW	4000
Complex of SARAH domains from RASSF5 and MST2	RASSF5MST2 SARAH	Q8WWW0 and Q13188	12.3	5, 6, 11, 12, 18, 24, 36, 44, 48, 52, 60, 62, 64, 67, 71, 74, 76, 84, 87, 92, 93	LMW, MMW, HMW, BMW	3350
Bloom syndrome protein	BLMnanobody complex	P54132	92.1	79	HMW	20K
Cullin-3 and Kelch-like protein 11	CUL3KLHL11 complex	Q13618 and Q9NVR0	77.3	10, 15, 22, 43, 56, 68, 70, 74	LMW, MMW, HMW, BMW	3350, 10K
Bromodomain-containing protein 9 (bromodomain)	BRD9	Q9H8M2	14.2	5, 11, 34, 45, 90	LMW, MMW, HMW, BMW	1K, 2K MME, 3350, 6K, 8K
Bromodomain and WD repeat-containing protein 1 (bromodomain)	WDR9	Q9NSI6	14.4	4, 5	LMW	3350