Table 2. Dependence of NowGFP green fluorescence (abs max/em max = 493/502nm) on pH.
| pH | EC† (M 1cm1) | QY‡ | B§ |
|---|---|---|---|
| 4.9 | 2700 | 0.71 | 1.9 |
| 5.5 | 6900 | 0.90 | 7.1 |
| 6.5 | 38300 | 0.73 | 28.0 |
| 7.1 | 53600 | 0.68 | 36.4 |
| 9.1 | 61300 | 0.68 | 41.7 |
EC, molar extinction coefficient at 493nm.
QY, fluorescence quantum yield.
B, brightness: (EC QY)/1000.