Table 3. Alternative conformations of side chains of the residues in the chromophore environment.
The alternative values for the 1 or 1/2 torsion angles are separated by sign, and the percentages of the alternative conformational states are given in parentheses. Cerulean has Val at position 61.
| Torsion angles (): 1 (CC) or 1/2 (CC/CC) | ||||
|---|---|---|---|---|
| NowGFP | Cerulean | |||
| Residue | pH 9.0 | pH 4.8 | pH 7† | pH 5‡ |
| Trp66 (chromophore) | 4/5 (cis) | 1/7 (80% cis) and 2/167 (20% trans) | 5/6 (cis) | 1/180 (trans) |
| Thr65 (chromophore) | 180 (70%) and 62 (30%) | 179 (50%) and 59 (50%) | 176 | 58 |
| Lys61 | 78/172 (20%) and 166/109 (80%) | 81/177 (80%) and 166/156 (20%) | ||
| Ser205 | 173 | 172 | 54 | 105 |
| Leu220 | 70/179 | 61/175 | 69/174 and 169/154 | 166/58 |