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. 2015 Aug 7;8:55. doi: 10.1186/s13048-015-0184-9

Fig. 2.

Fig. 2

Isolation of OSCs. Representative procedure of OSC separation from ovarian cortex, incubation with rabbit anti-Ddx4 antibody, subsequent incubation with anti-rabbit IgG conjugated with magnetic beads, capture of cells by magnet and further separation through MACS column leading to collection of Ddx4+ cells, detachment form the beads and elution in medium to obtain enriched OSC preparations