Table 1. Macromolecule-production information.

Source organism	M. tuberculosis H37Rv
DNA source	M. tuberculosis genomic DNA (obtained from BEI Resources)
Forward primer†	ATATATGCTAGCATGGAGGTTTCCGAATTCGAGGC
Reverse primer‡	ATATATCTCGAGATTCCGGTGATGCCTGCC
Cloning vector	Modified pET-15b§
Expression vector	Modified pET-15b
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSSGLVPRGSHMASMEVSEFEALRQHLMSVAYRLTGTVADAEDIVQEAWLRWDSPDTVIADPRAWLTTVVSRLGLDKLRSAAHRRETYTGTWLPEPVVTGLDATDPLAAVVAAEDARFAAMVVLERLRPDQRVAFVLHDGFAVPFAEVAEVLGTSEAAARQLASRARKAVTAQPALISGDPDPAHNEVVGRLMAAMAAGDLDTVVSLLHPDVTFTGDSNGKAPTAVRAVRGSDKVVRFILGLVQRYGPGLFGANQLALVNGELGAYTAGLPGVDGYRAMAPRITAITVRDGKVCALWDIANPDKFTGSPLKERRAQPTGRGRHHRNLE

^†Contains an NheI site.

^‡Contains an XhoI site.

^§The vector has been modified to contain an additional restriction site (NheI) and a stop codon (after XhoI).