Table 2. Crystallization.
| Method | Sitting-drop vapour diffusion |
| Plate type | Corning 96-well protein crystallization plates |
| Temperature (K) | 291 |
| Protein concentration (mgml1) | 15 |
| Buffer composition of protein solution | 20mM phosphate pH 7.4 |
| Composition of reservoir solution | 36%(v/v) 2-methyl-2,4-pentanediol, 5%(w/v) polyethylene glycol 8000, 0.1M sodium cacodylate pH 6.5 |
| Volume and ratio of drop | 1l drops, 1:1 ratio |
| Volume of reservoir (l) | 80 |