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. 2015 Jul 28;71(Pt 8):962–965. doi: 10.1107/S2053230X15009978

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type Corning 96-well protein crystallization plates
Temperature (K) 291
Protein concentration (mgml1) 15
Buffer composition of protein solution 20mM phosphate pH 7.4
Composition of reservoir solution 36%(v/v) 2-methyl-2,4-pentanediol, 5%(w/v) polyethylene glycol 8000, 0.1M sodium cacodylate pH 6.5
Volume and ratio of drop 1l drops, 1:1 ratio
Volume of reservoir (l) 80