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. 2015 Jul 28;71(Pt 8):993–997. doi: 10.1107/S2053230X15010195

Table 1. Macromolecule-production information.

Source organism O. aries
DNA source O. aries cDNA
Forward primer 5-CAGGGACCCGGTATGGACTCCTTGCCG-3
Reverse primer 5-CGAGGAGAAGCCCGGTTATAACGTATCCACT-3
Cloning vector pET-28 plasmid
Expression vector pET-28 plasmid
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the full-length construct produced (galectin-111140) MAHHHHHHSAALEVLFQGPGMDSLPNPYLQSVSLTVCYMVKIKANLLSPFGKNPELQVDFGTGTGQGGDIPFRFWYCDGIVVMNTLKDGSWGKEQKLHTEAFVPGQPFELQFLVLENEYQVFVNNKPICQFAHRLPLQSVKMLDVRGDIVLTSVDTL
Amino-acid sequence of crystallized galectin-11 after HCV-3C protease treatment (galectin-1118140) GPGMDSLPNPYLQSVSLTVCYMVKIKANLLSPFGKNPELQVDFGTGTGQGGDIPFRFWYCDGIVVMNTLKDGSWGKEQKLHTEAFVPGQPFELQFLVLENEYQVFVNNKPICQFAHRLPLQSVKMLDVRGDIVLTSVDTL