Table 1. Macromolecule-production information.
Source organism | O. aries |
DNA source | O. aries cDNA |
Forward primer | 5-CAGGGACCCGGTATGGACTCCTTGCCG-3 |
Reverse primer | 5-CGAGGAGAAGCCCGGTTATAACGTATCCACT-3 |
Cloning vector | pET-28 plasmid |
Expression vector | pET-28 plasmid |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the full-length construct produced (galectin-111140) | MAHHHHHHSAALEVLFQGPGMDSLPNPYLQSVSLTVCYMVKIKANLLSPFGKNPELQVDFGTGTGQGGDIPFRFWYCDGIVVMNTLKDGSWGKEQKLHTEAFVPGQPFELQFLVLENEYQVFVNNKPICQFAHRLPLQSVKMLDVRGDIVLTSVDTL |
Amino-acid sequence of crystallized galectin-11 after HCV-3C protease treatment (galectin-1118140) | GPGMDSLPNPYLQSVSLTVCYMVKIKANLLSPFGKNPELQVDFGTGTGQGGDIPFRFWYCDGIVVMNTLKDGSWGKEQKLHTEAFVPGQPFELQFLVLENEYQVFVNNKPICQFAHRLPLQSVKMLDVRGDIVLTSVDTL |