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| Method | Hanging drop |
| Plate type | 24-well Linbro plate |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 5 |
| Buffer composition of protein solution | 10mM TrisHCl, 125mM NaCl, 5mM TCEP, 10%(v/v) glycerol |
| Composition of reservoir solution | 2%(v/v) Tacsimate pH 7.0, 0.1M HEPES pH 7.5, 20%(w/v) PEG 3350 |
| Volume and ratio of drop | 1l protein solution:1l reservoir solution |
| Volume of reservoir (l) | 500 |
