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. 2015 Jul 29;71(Pt 8):1078–1082. doi: 10.1107/S2053230X15011759

Table 2. Crystallization.

  Apo VatD VatDFOB
Method Sitting-drop vapour diffusion Sitting-drop vapour diffusion
Plate type 96-well 96-well
Temperature (K) 293 293
Protein concentration (mgml1) 9 9
Buffer composition of protein solution 20mM Tris pH 7.5, 150mM NaCl 20mM Tris pH 7.5, 150mM NaCl
Composition of reservoir solution 0.1M Tris pH 7.0, 0.2M MgCl2, 2.5M NaCl 0.1M bis-tris pH 6.5, 50mM MgCl2, 30%(w/v) PEG 3350
Volume and ratio of drop 1.0l (1:1) 1.0l (1:1)
Volume of reservoir (l) 60 60