Table 2. Crystallization.
| Apo VatD | VatDFOB | |
|---|---|---|
| Method | Sitting-drop vapour diffusion | Sitting-drop vapour diffusion |
| Plate type | 96-well | 96-well |
| Temperature (K) | 293 | 293 |
| Protein concentration (mgml1) | 9 | 9 |
| Buffer composition of protein solution | 20mM Tris pH 7.5, 150mM NaCl | 20mM Tris pH 7.5, 150mM NaCl |
| Composition of reservoir solution | 0.1M Tris pH 7.0, 0.2M MgCl2, 2.5M NaCl | 0.1M bis-tris pH 6.5, 50mM MgCl2, 30%(w/v) PEG 3350 |
| Volume and ratio of drop | 1.0l (1:1) | 1.0l (1:1) |
| Volume of reservoir (l) | 60 | 60 |