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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2015 May 16;10(2):318–332. doi: 10.1007/s11481-015-9611-3

Figure 1.

Figure 1

O-1966 decreases levels of activated nuclear forms of NFκB and NFAT in T-cells. Splenocytes from CB2R k/o mice (open symbols) or wild-type mice (closed symbols) were treated for 3 h with O-1966 (WT: ●, k/o:○) or ethanol vehicle (WT: ■, k/o: □) and then added to a plate coated with 25 μg anti-CD3 antibody/well and soluble anti-CD28 antibody (0.4 μg/well) or left unstimulated (WT: ◆, k/o: ◇). The cultures were incubated for 18 h and cultures were harvested and nuclear proteins extracted. Levels of activated nuclear NFκB (Panel A) and NFAT (Panel B) were measured using a TransAM® Transcription Factor ELISA. Data are the mean ± S.E.M. of 3 separate experiments with triplicate wells for each treatment. *p < 0.01, **p < 0.001. (ANOVA, WT versus k/o). Values for vehicle are not significantly different from no treatment.