FIG. 2.

COL1A and alpha-smooth muscle actin (α-SMA) RNA content of (A, C) human cardiac fibroblasts (HCFs) and (B, D) normal human lung fibroblasts (NHLFs) cultured on cardiac extracellular matrix (ECM), lung ECM, and individual ECM components for 48 h, quantified by reverse transcription–quantitative polymerase chain reaction and normalized to expression of untreated fibroblasts (coll, type 1 collagen; FN, fibronectin; LECM1, lung ECM; CECM1, CECM2, and CECM3, cardiac ECM derived from three different conditions—see text for details). *Statistically significant differences between conditions (p<0.05), **statistically significant differences between conditions (p<0.01). N=3 for each gene. Error bars represent mean±standard deviation (SD). Color images available online at www.liebertpub.com/tea