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Reduced Nodal signaling in MZdchs1b embryos. (A) sqt expression in stage-matched WT and MZdchs1b embryos; animal-pole view. (B) Quantitative RT-PCR of sqt RNA in time-matched WT and MZdchs1b embryos during MBT. (C) Max z-projection of time-matched WT Tg[smad2-GFP] and MZdchs1b;Tg[smad2-GFP] embryos at 3.5 hpf. (D) Quantification of nuclear-to-cytoplasm ratio for GFP intensity in WT Tg[smad2-GFP] and MZdchs1b;Tg[smad2-GFP] embryos shown in C.
