Fig. 6.
Increased Ret signaling in Yap mutants and partial rescue of ND defects by Ret haploinsufficiency. (A-H) ISH revealed increased expression of Crlf1, Sprouty1, Etv4 and Etv5 expression in Yap mutant NDs (black arrowheads) compared with controls at E10.5. Note the apposition of the MM (arrows) on the UB (arrowheads) in Yap mutants, whereas both compartments are well separated in controls. (I-J″) Phospho-ERK staining in controls and Yap mutants at E11.5. E-cadherin counterstaining was used to label the epithelial structures. Panels J′ and J″ indicate the increased p-ERK expression in the CND (J′) and ND (J″) of Yap mutants compared with controls (I′). (K-N) Macroscopic views of the urogenital system from control (K), YapND−/− (L), Ret−/− (M) and YapND−/− Ret+/− (N) kidneys at P0. (O) Quantification of cases in which the ureter was connected to the bladder in controls and YapND−/− Ret+/− animals. Scale bars: 100 µm in A-J; 50 µm in I′-J″; 1 mm in K-N.