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. 2015 Aug 1;142(15):2641–2652. doi: 10.1242/dev.126318

Fig. 3.

Fig. 3.

Evf2 stabilizes BRG1 binding to key DNA regulatory enhancers in the Dlx5/6 intergenic region. (A) Mouse chromosome 6qA1 (Dlx5/6 region) showing relative location of q-PCR primers (1-4), Dlx5/6 enhancers (the ultraconserved enhancer ei and the conserved enhancer eii, red boxes) and Evf2, Evf1, Dlx5, Dlx6 transcripts (black arrows). Dlx5 and Dlx6 protein coding exons (black boxes). (B-G) ChIP of Evf2+/+ (yellow bars) and Evf2TS/TS (blue bars) E13.5 GE tissue is followed by q-PCR using primers at sites 1-4. Antibodies for ChIP are indicated on the y-axis. Primer 1, a site downstream of the 3′ end of Evf2; primers 2-4 are Dlx5/6 intergenic sites: primer 2, ei; primer 3, eii (Zerucha et al., 2000); primer 4, an adjacent intergenic region with no known regulatory role. *P<0.05, **P<0.01, ***P<0.001 (Student's two-tailed t-test), n=10 embryos each genotype; error bars indicate s.e.m. (H) Anti-BRG1 ChIP from E13.5 GE. q-PCR shows enrichment of BRG1 at a subset of UCR sites: the Dlx1 UCR site, but not the Ptc1 or Shh UCRs.