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Real-time PCR validated the upregulation of lysosomal transcripts in the SCNT blastocyst-stage embryos treated with HDACi observed by deep sequencing. A side-by-side comparison of deep sequencing results and real-time PCR are shown in panels A, B, C, D, E, and F for transcripts CTSK, CTSA, LGMN, HEXA, HEXB and SMPD1, respectively. Significant differences with a p value<0.05 are indicated by superscripts a, b, c, d. NT=SCNT.
