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. 2015 Aug 1;17(4):243–258. doi: 10.1089/cell.2015.0022

FIG. 4.

FIG. 4.

Lysosomes were localized by LysoTracker in IVV, IVF, SCNT (No HDACi), SCNT (ISAHA), SCNT (SAHA), and SCNT (Scriptaid) blastocyst-stage embryos. Two representative embryos from each treatment are shown in panels A–M. Representative negative controls are shown in panels G and M. CTSK localization is shown in panels O–T with a representative negative control is shown in panel U. Total cell number was determined by staining with the DNA Hoechst 33342 and counting nuclei. A representative embryo for each treatment is shown in panels V–AA with a negative control shown in panel BB. NT=SCNT.