Figure 4.

PFTβ treatment of mice results in increased numbers of HSCs in the BM. (A) 5 mice were given a single injection of PFTβ (25 mg kg⁻¹ body weight) or vehicle (“control”) without accompanying IR. BM-MNCs were isolated on day 5 after injection and stained for flow cytometric detection of HSCs (Lin, c-Kit⁺, Sca1⁺ “KSL” cells). Left: The total number of KSL cells per mouse (4 bones) is shown in the upper right quadrants of the scatter plots (representative results from individual control and PFTβ-treated mice). Right: Bar graph showing the mean number of KSL cells per mouse (4 bones) +/− SE (n = 5). (B) 7 mice were injected with PFTβ (25 mg kg⁻¹ body weight) or vehicle (“control”) without accompanying IR once a day for 5 days. BM-MNCs (top) and PBMCs (bottom) were isolated on day 6 and stained with Hoechst 33342. Side population (SP) cells equivalent to HSCs were detected by FACS using blue and red filters. Left: Representative scatter plots in which SP cells are shown gated and the number of cells per animal is indicated. Right: Bar graphs indicating the mean number of SP cells per animal +/− SE (n = 7).