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. 2015 Jun 24;21(15-16):2281–2291. doi: 10.1089/ten.tea.2015.0039

FIG. 5.

FIG. 5.

Analysis of osteogenic differentiation of CD55+ subpopulation. (A) Quantification of alkaline phosphatase stain intensity on ImageJ for unsorted and CD55− hASCs on day 7 of osteogenic differentiation (*p<0.01). (B) Quantification of Alizarin red S staining on photometric analysis of unsorted and CD55− hASCs on day 14 of osteogenic differentiation. Representative images of Alizarin red S staining on unsorted and CD55− hASCs at day 14 of osteogenic differentiation (*p<0.01). (C) qRT-PCR of OPN expression in unsorted and CD55− hASCs harvested from a single donor on day 0, 7, and 14 of osteogenic differentiation. Errors bars represent one standard deviation with an n=3 wells (*p and **p<0.01). (D) qRT-PCR of OCN expression in unsorted and CD55− hASCs harvested from a single donor on day 14 of osteogenic differentiation. Errors bars represent one standard deviation with an n=3 wells (*p and **p<0.01).