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PHF8 regulated bone transcription factors. The protein expressions of PHF8, Satb2, OSX, and Runx2 were detected by western blot. (A) PHF8 overexpression (left panel) and PHF8 suppression by shRNA (right panel) in MC3T3-E1 cells. (B, C) Quantification of protein levels. (D–F) The same experiments as (A–C) in BMSCs. *p<0.05.
