Fig 5. Characterization of erythroid cells from HSCs.

(A) Photographs of cytospin preparations of the erythroid cells. Cells from shSOCS3-1 or shcontrol CD34⁺ cells were stained by Wright-Giemsa on day 21 in liquid culture. The arrows directed enucleated cells. Bar = 50um. (B) Statistical analysis for the percent of enucleated cells in different groups. Enucleated cells on day 21 in liquid culture; the percent of enucleated cells was calculated as the ratio enucleated cells / all cells in a field. Data represent mean percent of positive cells ±SEM. n = 5, *p < 0.05. (C) Functional assays of erythroid cells from shSOCS3-1 CD34⁺ cells. Oxygen dissociation curves of RBCs derived from shSOCS3-1 CD34⁺ cells or human adult blood.