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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Atherosclerosis. 2015 Jun 18;241(2):709–715. doi: 10.1016/j.atherosclerosis.2015.06.018

Fig. 1.

Fig. 1

The anti-migratory effect of lysophosphatidylcholine (lysoPC) is attenuated in endothelial cells (EC) pretreated with the apoplipoprotein A-I mimetic peptide L-4F. Confluent bovine aortic EC (BAEC) were incubated with medium, L-4F (0.43 μmol/L), or scrambled peptide (0.43 μmol/L) for 3 hours prior to initiation of the migration assay. Migration was initiated, and lysoPC (12.5 μmol/L) was added as indicated during the migration assay. Migration was quantified after 24 hours. Arrow indicates starting line of migration. Original magnification, ×40. Bar, 100 μm. The bottom panel represents migration results by mean ± SD (n=3, * P<0.001 compared with medium control, ** P<0.001 compared with medium control and scrambled peptide control, *** P<0.01 compared with medium+lysoPC and with L-4F control). (A) EC were washed 3 times with PBS prior to the addition of lysoPC. (B) Pretreatment agents were not removed during incubation with lysoPC.