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. Author manuscript; available in PMC: 2015 Aug 7.
Published in final edited form as: Nat Med. 2012 Sep;18(9):1386–1393. doi: 10.1038/nm.2847

Figure 3.

Figure 3

NET-forming PMN display a novel crawling phenotype in vivo related to nuclear structure. (a) 2D images of in vivo PMN with a normal nuclei, NET-forming cells with diffuse nuclei and an in vitro generated anuclear human PMN (cytoplast). Contouring analysis of each cell is shown beneath the image. The PMN outer membrane is traced every 30 s (PMN in the middle image with white arrow is traced). Typical crawling phenotypes are shown for Tlr2−/− and C3−/− animals. (b) Cell polarities in relation to nuclear morphology were quantified. (c) The relationship of pseudopod formation of crawling PMN compared to their nuclear architecture or between wild type, Tlr2−/− and C3−/− mice. (d) PMN velocity and meandering index during live staphylococcus infection. (e) Cellular tracking during live staphylococcus infection (*** = P < 0.001, ** = P < 0.01, * = P < 0.05 compared to sterile inflammation and ### = P < 0.001, # = P < 0.05 compared to wild type).