Table 3. HIV-specific IFN-γ ELISPOT Responses, Magnitude and Breadth after HIVMAG +/-IL-12 and Ad35 GRIN/Env.
| HIV-MAG peptide pools* | GRIN-Env peptide pools* | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Group | N/Total | Priming Regimen | Mean (SE) | GM (95% CI) | Breadth | N/Total | Boosting Regimen | Mean (SE) | GM (95% CI) | Breadth |
| 1 | 8/11 | HIVMAG (x3) | 337 (114) | 227 (102–506) | 3.0 | 8/11 | Ad35 GRIN/Env (x1) | 620 (172) | 418 (171–1025) | 3.6 |
| 2 | 7/11 | HIVMAG + 100μg IL-12 (x3) | 391 (141) | 234 (78–706) | 2.9 | 8/11 | Ad35 GRIN/Env (x1) | 172 (41) | 142 (82–246) | 1.4 |
| 3 | 5/12 | HIVMAG + 1000μg IL-12 (x3) | 155 (35) | 135 (61–299) | 1.8 | 8/9 | Ad35 GRIN/Env (x1) | 269 (72) | 203 (102–403) | 2.1 |
| 4 | 0/8 | HIVMAG + 1000μg IL-12 (x1) | --- | --- | --- | 4/10 | Ad35 GRIN/Env (x1) | 253 (56) | 227 (91–569) | 1.8 |
| 5 | 6/12 | Ad35 GRIN/Env (x1) | 149 (41) | 117 (50–273) | 2.0 | 3/11 | HIVMAG + 1000μg IL-12 (x1) | 129 (51) | 110 (19–630) | 2.3 |
*Sum of all background-subtracted positive responses for each volunteer over the 6 HIV-MAG or GRIN/Env peptide pools. N = Number of positive assays. GM = Geometric Mean. CI = Confidence Interval. SE = Standard Error. Breadth = Mean number of positive peptide pools per volunteer.