Table 1. List of primers used in the study.
Primer code | Sequence (5′-3′) | Direction | Application |
---|---|---|---|
CamCPR-F | CA(A/G)AC(C/T)GGIACIGC(C/T)GA(A/G) G | Forward | Degenerate primers |
CamCPR-R | TT(A/G)GC(A/G)TCACC(A/G)CAIACATA | Reverse | |
AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | Forward | 5′-RACE |
5GSP1 | TCATACTGCCTGTTGCCGAGAC | Reverse | |
5GSP2 | CCATCTCCGTATGTAGCTAAGAAG | Reverse | |
3GSP1 | TACCGTCGTTCCACTAGTGATTT | Forward | 3′-RACE |
3RACE Outer Primer | TACCGTCGTTCCACTAGTGATTT | Reverse | |
CamCPR-Full-F | CGGTAAGATGCAATCGAGTTCG | Forward | Full length cloning |
CamCPR-Full-R | GAATTGGCAGCGAAGGTGAGTA | Reverse | |
His6-CamCPR-F | GGAATTCCATATGCAATCGAGTTCGG | Forward | Overexpression |
His6-tCamCPR-F | GGAATTCCATATGTCGTCAGGAAAGTC | Forward | |
His6-CamCPR-R | CCGCTCGAGTCACCACACATCACGC | Reverse | |
CamCPR-His6-F | CATGCCATGGCATCGAGTTCGGTTAAG | Forward | |
tCamCPR-His6-F | GGAATTCCATATGTCGTCAGGAAAGTC | Forward | |
CamCPR-His6-R | CCCAAGCTTCCACACATCACGCAAATACC | Reverse | |
rtCamCPR-F | TGAAACTGGTGCACTCTCTGAG | Forward | Quantitative real-time PCR analysis |
rtCamCPR-R | CAGTCTTGGAGCTGTCTAGAGATC | Reverse | |
rtActin-F | TTCCTCATGCCATCCTCCGTCTT | Forward | |
rtActin-R | CAGCGATACCTGAGAACATAGTGG | Reverse |