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. 2015 Aug 7;10(8):e0135397. doi: 10.1371/journal.pone.0135397

Table 1. List of primers used in the study.

Primer code Sequence (5′-3′) Direction Application
CamCPR-F CA(A/G)AC(C/T)GGIACIGC(C/T)GA(A/G) G Forward Degenerate primers
CamCPR-R TT(A/G)GC(A/G)TCACC(A/G)CAIACATA Reverse
AAP GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG Forward 5′-RACE
5GSP1 TCATACTGCCTGTTGCCGAGAC Reverse
5GSP2 CCATCTCCGTATGTAGCTAAGAAG Reverse
3GSP1 TACCGTCGTTCCACTAGTGATTT Forward 3′-RACE
3RACE Outer Primer TACCGTCGTTCCACTAGTGATTT Reverse
CamCPR-Full-F CGGTAAGATGCAATCGAGTTCG Forward Full length cloning
CamCPR-Full-R GAATTGGCAGCGAAGGTGAGTA Reverse
His6-CamCPR-F GGAATTCCATATGCAATCGAGTTCGG Forward Overexpression
His6-tCamCPR-F GGAATTCCATATGTCGTCAGGAAAGTC Forward
His6-CamCPR-R CCGCTCGAGTCACCACACATCACGC Reverse
CamCPR-His6-F CATGCCATGGCATCGAGTTCGGTTAAG Forward
tCamCPR-His6-F GGAATTCCATATGTCGTCAGGAAAGTC Forward
CamCPR-His6-R CCCAAGCTTCCACACATCACGCAAATACC Reverse
rtCamCPR-F TGAAACTGGTGCACTCTCTGAG Forward Quantitative real-time PCR analysis
rtCamCPR-R CAGTCTTGGAGCTGTCTAGAGATC Reverse
rtActin-F TTCCTCATGCCATCCTCCGTCTT Forward
rtActin-R CAGCGATACCTGAGAACATAGTGG Reverse