Table 1. Sense sequences of the oligonucleotides used in EMSA.
Name | Sequence 5′ → 3′ |
---|---|
AP1 (WT) | TACAGGCGTGAGTCACTGCGCCCGGCCCGCA |
mAP1 | TACAGGCGaGctcCACTGCGCCCGGCCCGCA |
CF1-1(WT) | ATGGGGACCATGTGGTCACTGAAGTCCTA |
mCF1-1 | ATGGGGAttcTGcGGTCACTGAAGTCCTA |
CF1-2(WT) | TGTCACCATATTGGCCAGGCTGGTCT |
mCF1-2 | TGTCAttcTATTGGCCAGGCTGGTCT |
C/EBPβ-1(WT) | AGTGGTGCAATCTCGGCTCACTGCGA |
mC/EBPβ-1 | AGTGGTaaccaCTCGGCTCACTGCGA |
C/EBPβ-2(WT) | GAGCACCTGCAGGAATTTTTTAAGGGGATG |
mC/EBPβ-2 | GAGCACCTatcaGTcTTTTTTAAGGGGATG |
Sp1-1+2(WT) | GAGTGTGGGCGGGGGCTGGTGCCTGGAGGGGCCTTC |
mSp1-2 | GAGTGTGGGCGGGGGtctGTGCCTGGAGGGGCCTTC |
mSp1-1+2 | GAGTGTGttgGGGGGtctGTGCCTGGAGGGGCCTTC |
Sp1-3(WT) | TGATCCGCCCGCCTTGGCCTCCCAAAGT |
mSp1-3 | TGATCCGCCCtaCTTGGCCTCCCAAAGT |
Mutated nucleotides are indicated in lowercase letters. WT – wild-type oligonucleotide. m – mutant oligonucleotide.