Table 2. Oligonucleotide pairs used for making the mutant luciferase reporter constructs.
| Name | Sequence change | Sequence a | Position b | |
|---|---|---|---|---|
| Sense (5′ to 3′) | Antisense (5′ to 3′) | |||
| mSp1-1 | GGC to ttg | GAGTGTGttgGGGGGCTGGTGCCTGGAGGGGCCTTC | GAAGGCCCCTCCAGGCACCAGCCCCCcaaCACACTC | -646/-611 |
| mSp1-2 | CTG to tct | GAGTGTGGGCGGGGGtctGTGCCTGGAGGGGCCTTC | GAAGGCCCCTCCAGGCACagaCCCCCGCCCACACTC | -646/-611 |
| mSp1-1+2 | GGC to ttg CTG to tct | GAGTGTGttgGGGG GtctGTGCCTGGAGGGGCCTTC | GAAGGCCCCTCCAGGCACagaCCCCCcaaCACACTC | -646/-611 |
| mSp1-3 | GC to ta | TGATCCGCCCtaCTTGGCCTCCCAAAGT | ACTTTGGGAGGCCAAGtaGGGCGGATCA | -320/-293 |
| mAP1 | T to a AGT to ctc | TACAGGCGaGctcCACTGCGCCCGGCCCGCA | TGCGGGCCGGGCGCAGTGgagCtCGCCTGTA | -284/-254 |
| mCF1-1 | CCA to ttc, T to c | ATGGGGAttcTGcGGTCACTGAAGTCCTA | TAGGACTTCAGTGACCgCAgaaTCCCCAT | -593/-565 |
| mCF1-2 | CCA to ttc | TGTCAttcTATTGGCCAGGCTGGTCT | AGACCAGCCTGGCCAATAgaaTGACA | -362/-337 |
| mC/EBPβ-1 | GCAAT to aacca | AGTGGTaaccaCTCGGCTCACTGCGA | TCGCAGTGAGCCGAGtggttACCACT | -499/-474 |
| mC/EBPβ-2 | GCAG to atca, AA to tc | GAGCACCTatcaGtcTTTTTTAAGGGGATG | CATCCCCTTAAAAAAgaCtgatAGGTGCTC | -240/-211 |
a Putative transcription factor binding sites are underlined (one consensus sequence in Sp1-1+2 is bolded) and mutated nucleotides are indicated in lowercase letters. Single-stranded oligonucleotides were synthesized by Genomed S.A.
b Position of oligonucleotides relative to the transcriptional start site (+1).