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. 2015 Aug 7;10(8):e0134176. doi: 10.1371/journal.pone.0134176

Fig 1. DT induced pDC depletion does not influence atherosclerotic lesion size, lesional macrophage numbers and plasma lipid levels in Apoe -/- BDCA2-DTR mice after 4 weeks of HFD.

Fig 1

(A) Depletion efficiency of pDCs after i.p. DT administration in Apoe −/− mice (control) compared to Apoe −/− BDCA2-DTR mice (pDC depleted). Representative dot plots show B220+mPDCA-1+440c+ pDC populations as percentage of CD45+ cells in spleen after 24 and 48 hours (one single i.p. injection) and one week (3 times i.p. injection) after DT administration. (B) Quantification of atherosclerotic lesion size with Oil Red O+ area (% of aortic root surface) and (C) Lesional MAC2+ macrophages (% of DAPI+ cells) in aortic root cryosections of Apoe −/− and Apoe −/− BDCA2-DTR mice after 4 weeks of HFD (scale bar = 500 μm). (D) Plasma cholesterol and triglycerides levels (mg/dL) of Apoe −/− and Apoe −/− BDCA2-DTR mice after 4 weeks of HFD. Graphs represent the mean±SD; n = 6 to n = 10.