Table 1. Functional characteristics of the ASIC1a constructs.
| wt | ΔCCt | V74C | Y426C | G430C | G433C | |
|---|---|---|---|---|---|---|
| INa (μA) | 55.33±26.88 | 45.06±20.6 | 2.9±2.4** | 22.65±14.22 | 22.75±14.73 | 3.6±3.8 ** |
| pH0.5 | 6.35±0.02 | 6.35±0.014 | 6.36±0.04 | 6.57±0.01 | 6.36±0.01 | 6.55±0.02 |
| IC50 (μM) amiloride | 219±43 | 150±24 | 7.1±1.4 | 31.9±4.2 | 35.3±4.9 | 2.5±0.51 |
| IC50 Cd2+ (mM) | 0.527±0.049 | 1.16±0.095 | < 0,01 | 0.032±0.0016* | 0.010±0.001* | 0.057±0.0037* |
| INa + BMOE (% of control) | ND | 90.2±10.0 | 115.7±24 | 6.4±0.18** | 9.5±0.17** | 89.1±13.3 |
| INa (μA) +DTT | ND | 38.04±15.24 | 25.84±15.72 | 29.92±16.8 | 26.29±19.37 | 3.79±2.92 |
ASIC1a currents (INa) were elicited at pH 6.0. ΔCCt denotes ASIC1a mutant lacking the cysteines in the C-terminus. BMOE (2mM), Cd2+ and DTT (10 mM) were added in the extracellular medium.
** denotes statistical significance p<0.01 and
* p<0.05, unpaired t-test for the mutant compared to ASIC1a wt. denotes p<0.01 for treated versus untreated condition.
Current values (μA or % of control) are expressed as mean ± SD
IC0.5 and pH0.5 are expressed as best-fit values ± 95% confidence intervals of at least 4 independent experiments