Fig 4. Median-joining networks.

Haplotype relationships are depicted within each of the four genealogical lineages of the causal agents of Cercospora leaf blight and purple seed stain of soybean. Networks were based on a dataset containing two nuclear gene regions concatenated: the cercosporin facilitator protein (cfp) and the β-tubulin 1 (tub) genes. In each network, a circle represents a given haplotype (coded with numbers); size is proportional to the relative frequencies. Numbers of mutational steps are indicated with bars when there is more than one (unless indicated otherwise). Color codes according to the origin of the sequences: blue, Argentina; green, Brazil; and red, United States. Diamonds indicate haplotypes harboring the mutation E198A, which is known to confer resistance to benzimidazole fungicides. In Lineage 1, an arrow denotes the location of the haplotype of CPC 5068, the ex-type strain of C. kikuchii.