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. 2015 Jul 31;9:3989–4104. doi: 10.2147/DDDT.S85426

Figure 11.

Figure 11

Effect of UA on the expression of TIMP-1 and MMP-1 in HSC-T6 cells.

Notes: Cells were pretreated with UA (50 µM), DPI (20 µM), SB203580 (10 µM), or LY294002 (10 µM) for 30 minutes and then stimulated with leptin for another 24 hours. The expression level of TIMP-1 and MMP-1 was examined using Western blotting assays. (A) Representative blots of TIMP-1 and MMP-1 in HSC-T6 cells. (B) Bar graphs showing the relative level of TIMP-1 and MMP-1 in HSC-T6 cells. Data are expressed as mean ± SD from six independent experiments. β-Actin acts as an internal control. *P<0.05; ***P<0.001. + and − represent with or without treatment, respectively.

Abbreviations: UA, ursolic acid; TIMP-1, TIMP metallopeptidase inhibitor 1; MMP-1, matrix metalloproteinase-1; HSC, hepatic stellate cell; DPI, diphenyleneiodonium; SD, standard deviation.