Figure 12.

Effect of UA on the mRNA expression of collagen I in HSC-T6 cells.

Notes: Cells were pretreated with UA (50 µM), DPI (20 µM), AG490 (50 µM), or PD98059 (30 µM) for 30 minutes and then stimulated with leptin (100 ng/mL) for another 24 hours. The mRNA expression level of type I collagen I was examined using PCR assays. (A) Representative blots of type I collagen I in HSC-T6 cells. (B) Bar graph showing the relative level of type I collagen I in HSC-T6 cells. Data are expressed as mean ± SD from six independent experiments. β-Actin acts as an internal control. *P<0.05; **P<0.01. + and − represent with or without treatment, respectively.

Abbreviations: UA, ursolic acid; HSC, hepatic stellate cell; DPI, diphenyleneiodonium; PCR, polymerase chain reaction; SD, standard deviation.