Figure 8.

Effect of UA on the expression of NOX subunits of HSC-T6 cells.

Notes: Cells were pretreated with UA (50 µM) for 30 minutes and then stimulated with leptin (100 ng/mL) for another 12 hours. The expression level of gp91^phox, p22^phox, p67^phox, and Rac1 was examined using Western blotting assay. (A) Representative blots of gp91^phox, p22^phox, p67^phox, and Rac1 in HSC-T6 cells. (B) Bar graphs showing the relative level of gp91^phox, p22^phox, p67^phox, and Rac1 in HSC-T6 cells. Data are expressed as mean ± SD from six independent experiments. β-Actin acts as an internal control. **P<0.01; ***P<0.001.

Abbreviations: UA, ursolic acid; NOX, NADPH oxidase; HSC, hepatic stellate cell; SD, standard deviation.