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. 2015 Aug 7;10(8):e0134692. doi: 10.1371/journal.pone.0134692

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© 2015 Ferreira et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — The data presented show the activity of each live Pa isolate or planktonic or biofilm generated culture filtrate on preformed biofilm of Af. Individual isolate results are shown in comparison with their respective Af controls (n = 6 for each). There were five non-CF, five mucoid CF and five non-mucoid CF isolates tested; isolate designations are shown on the x-axis. The bars on the far right of each panel represent the pooled data for each isolate (n = 30). Bars represent the mean ± SD of the XTT reduction at read at 490 nm. Panel (A) Af preformed biofilms exposed to live Pa cells for 24 h. (B) Af preformed biofilms were exposed to Pa planktonic spent supernatant for 24 h. (C) Af preformed biofilms were exposed to Pa biofilm spent supernatant for 24 h. Each data point represents the XTT metabolic activity obtained spectrophotometrically at 490 nm. Assays were performed in triplicate and the results are pooled from two experiments for each Pa-Af combination. The asterisk indicates a significant P value (< 0.001) for the XTT metabolic activity compared to the positive control, using the same analytic methodology as in Fig 2. Panel (A) Individual Pa isolate comparisons showed that only a one non-CF isolate, Pa19, inhibited (P<0.01), whereas all mucoid or non-mucoid CF isolates were inhibitory (P<0.001, all comparisons). Pooled data analysis showed that both mucoid and non-mucoid CF isolates were significantly inhibitory (P<0.001, both comparisons) and that non-mucoid CF isolates were more inhibitory than non-CF or mucoid Pa isolates (P< 0.001, both comparisons). Panel (B) Planktonic spent medium from a single non-CF isolate, Pa19, was inhibitory (P<0.05), whereas all mucoid and non-mucoid CF were significantly inhibitory (P < 0.001). Pooled data analysis showed that planktonic culture filtrate from CF non-mucoid or mucoid isolates was inhibitory (P<0.001, both comparisons). Non-mucoid CF isolates were more inhibitory than mucoid CF isolates (P<0.001). Panel (C) Biofilm culture filtrate from non-CF isolates of Pa was not inhibitory, whereas the culture filtrate from each mucoid or non-mucoid CF Pa isolates was inhibitory (P< 0.001, all comparisons). Pooled data analysis showed that biofilm culture filtrate from CF non-mucoid or mucoid isolates was inhibitory (P<0.001). Non-mucoid CF isolate filtrates were more inhibitory than mucoid CF isolates (P<0.001).